Changes in radiation sensitivity of human osteosarcoma cells after p53 introduction

J Miyakoshi; N Yamagishi; S Ohtsu; H Takebe

doi:10.1111/j.1349-7006.1995.tb02457.x

Changes in radiation sensitivity of human osteosarcoma cells after p53 introduction

Jpn J Cancer Res. 1995 Aug;86(8):711-3. doi: 10.1111/j.1349-7006.1995.tb02457.x.

Authors

J Miyakoshi¹, N Yamagishi, S Ohtsu, H Takebe

Affiliation

¹ Department of Radiation Genetics, Faculty of Medicine, Kyoto University.

Abstract

Human osteosarcoma SAOS-2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSVneop53 containing human p53 cDNA and neomycin-resistance gene. Three clones (SAOS-MC10, SAOS-MC11 and SAOS-MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS-MC10, while SAOS-MC11 and SAOS-MC43 produced p53 protein. The molecular weight of p53 protein in SAOS-MC43 was lower than that in SAOS-MC11, SAOS-MC11 and SAOS-MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS-2. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bone Neoplasms / genetics*
Bone Neoplasms / pathology*
Bone Neoplasms / radiotherapy
Genes, p53 / physiology*
Humans
Osteosarcoma / genetics*
Osteosarcoma / pathology*
Osteosarcoma / radiotherapy
Radiation Tolerance
Transfection*
Tumor Cells, Cultured / radiation effects