Abstract
The gene encoding the major autolysin of Staphylococcus aureus 8325/4 has been cloned, sequenced, and insertionally inactivated. The three-domain, 137,384-Da protein has a C-terminal glucosaminidase active site and is involved in cell separation, generalized cell lysis, and release of wall material at the cell surface. Expression occurs throughout growth and is stimulated by low temperatures and in the presence of 1 M NaCl.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Binding Sites
-
Cell Wall / enzymology
-
Cloning, Molecular
-
Gene Expression Regulation, Bacterial / drug effects
-
Genes, Bacterial / genetics*
-
Hexosaminidases / metabolism
-
Molecular Sequence Data
-
Mutation
-
N-Acetylmuramoyl-L-alanine Amidase / genetics
-
N-Acetylmuramoyl-L-alanine Amidase / metabolism*
-
Recombinant Fusion Proteins / biosynthesis
-
Restriction Mapping
-
Sequence Analysis, DNA
-
Sodium Chloride / pharmacology
-
Staphylococcus aureus / enzymology*
-
Staphylococcus aureus / genetics
-
Temperature
Substances
-
Recombinant Fusion Proteins
-
Sodium Chloride
-
Hexosaminidases
-
N-Acetylmuramoyl-L-alanine Amidase