Bartonella henselae was isolated from the blood of five febrile immunosuppressed patients using BacT/Alert (Organon Teknika, Durham, NC) automated microbial detection system. An immunofluorescence assay (using 1:1000 dilutions) was used to confirm identification of fastidious, pleomorphic, non-Gram staining, argyrophilic bacilli displaying rachety motility that had been presumptively identified as Bartonella spp. The practicality of identification of Bartonella henselae using goat antisera for use in a routine clinical microbiology laboratory was demonstrated by this study.