The extraction and purificatory crafts for Radix Sophorae Flavescentis were observed through the orthogonal experiment design using matrine as the fixed indicatrix for TLC-scanning. Quantitative analyses of matrine were made on the momentum parameters of the different craft conditions. The results of the three different extraction methods show that the best way is to use 2% gelatin to remove tannin with the least loss of matrine, and that the ethanol extract from water precitation is better than the water extract from ethanol precipitation.