Abstract
Synthetic peptides corresponding to microbial epitopes stimulate T cell immunity but their immunogenicity is poor and their half-lives are short. A viral epitope inserted into the complementarity-determining region 3 (CDR3) loop of the heavy chain of a self immunoglobulin (Ig) molecule was generated from the Ig context and was presented by I-Ed class II molecules to virus-specific, CD4+ T cells. Chimeric Ig-peptide was presented 100 to 1000 times more efficiently than free synthetic peptide and was able to prime virus-specific T cells in vivo. These features suggest that antigenized Ig can provide an improved and safe vaccine for the presentation of microbial and other peptides.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigen-Presenting Cells / immunology*
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Antigens, Viral / immunology*
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Arsenic / immunology
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Arsenicals*
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Base Sequence
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CD4-Positive T-Lymphocytes / immunology
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DNA / genetics
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Epitopes / immunology*
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Hemagglutinin Glycoproteins, Influenza Virus
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Hemagglutinins, Viral / genetics
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Hemagglutinins, Viral / immunology
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Histocompatibility Antigens Class II / immunology
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Heavy Chains / immunology
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Immunoglobulin Variable Region / genetics
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Immunoglobulin Variable Region / immunology
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Immunoglobulins / genetics
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Immunoglobulins / immunology*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Mutagenesis
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Receptors, Fc / immunology
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Recombinant Fusion Proteins / immunology
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Transfection
Substances
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Antigens, Viral
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Arsenicals
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Epitopes
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Hemagglutinin Glycoproteins, Influenza Virus
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Hemagglutinins, Viral
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Histocompatibility Antigens Class II
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Immunoglobulin Heavy Chains
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Immunoglobulin Variable Region
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Immunoglobulins
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Receptors, Fc
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Recombinant Fusion Proteins
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DNA
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arsonic acid
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Arsenic