Abstract
Recombinant human macrophage inflammatory protein-1 alpha (rhMIP-1 alpha) and rhMIP-1 beta were potent chemoattractants of human T lymphocytes. These rhMIP-1 cytokines attracted only T cells activated by monoclonal antibody to CD3 and did not attract unstimulated lymphocytes. Phenotypic analysis revealed that CD4+ T cells were capable of migrating in response to rhMIP-1 beta, whereas rhMIP-1 alpha induced chemotaxis of predominantly CD8+ T lymphocytes. Activated naïve and memory T cells also migrated in response to rhMIP-1 cytokines. Furthermore, these cytokines enhanced the ability of T cells to bind to an endothelial cell monolayer. These results suggest that rhMIP-1 cytokines preferentially recruit specific T cell subsets during the evolution of the immune response.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adult
-
Antibodies, Monoclonal / immunology
-
CD3 Complex / immunology
-
CD4-Positive T-Lymphocytes / immunology
-
CD4-Positive T-Lymphocytes / physiology*
-
CD8 Antigens / analysis
-
Cell Adhesion
-
Chemokine CCL4
-
Chemokine CCL5
-
Chemotaxis, Leukocyte*
-
Clone Cells
-
Cytokines / pharmacology*
-
Endothelium, Vascular / cytology
-
Humans
-
Immunologic Memory
-
Lymphocyte Activation*
-
Lymphokines / pharmacology
-
Macrophage Inflammatory Proteins
-
Monokines / pharmacology*
-
Recombinant Proteins / pharmacology
-
T-Lymphocyte Subsets / immunology
-
T-Lymphocyte Subsets / physiology
-
T-Lymphocytes, Cytotoxic / immunology
-
T-Lymphocytes, Cytotoxic / physiology*
-
T-Lymphocytes, Regulatory / immunology
-
T-Lymphocytes, Regulatory / physiology*
-
Umbilical Veins
Substances
-
Antibodies, Monoclonal
-
CD3 Complex
-
CD8 Antigens
-
Chemokine CCL4
-
Chemokine CCL5
-
Cytokines
-
Lymphokines
-
Macrophage Inflammatory Proteins
-
Monokines
-
Recombinant Proteins