Direct characterization of bovine microsatellites from cosmids: polymorphism and synteny mapping

Anim Genet. 1994 Aug;25(4):209-14. doi: 10.1111/j.1365-2052.1994.tb00195.x.

Abstract

A (TG)8 oligonucleotide probe was used to screen 186 cosmids from a commercial bovine cosmid library. Of the 56 positive discovered, 7 were sequenced in the region of the microsatellite and analysed for polymorphism. These microsatellites, IDVGA-2, -3, -7, -8, -9, -10, -11 showed the following number of alleles and polymorphism information content (PIC) values (7/0.616, 8/0.693, 6/0.641, 5/0.643, 2/0.239, 10/0.844, 6/0.720). The microsatellites were also assigned to synteny groups as follows: IDVGA-2/U17, IDVGA-3/U16, IDVGA-7/U7, IDVGA-8/U29, IDVGA-9/U3, IDVGA-10/U19, IDVGA-11/A (probably U18).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cattle / genetics*
  • Chromosome Mapping / veterinary*
  • Cosmids*
  • DNA Primers
  • DNA, Satellite*
  • Female
  • Male
  • Molecular Sequence Data
  • Pedigree
  • Polymorphism, Genetic*

Substances

  • DNA Primers
  • DNA, Satellite

Associated data

  • GENBANK/Z27071
  • GENBANK/Z27072
  • GENBANK/Z27073
  • GENBANK/Z27074
  • GENBANK/Z27075
  • GENBANK/Z27076
  • GENBANK/Z27077