Fluoride prevents degranulation of the azurophilic and specific granules in electropermeabilized neutrophils

J Leukoc Biol. 1994 Apr;55(4):489-95. doi: 10.1002/jlb.55.4.489.

Abstract

The effect of AlF4- on the degranulation process in human neutrophils was investigated. In intact neutrophils, AlF4- induced degranulation, whereas in electropermeabilized neutrophils AlF4- did not stimulate degranulation. In electropermeabilized neutrophils, fluoride ions proved to be inhibitory for the degranulation induced by addition of Ca2+ and/or GTP-gamma-S. Another phosphatase inhibitor, okadaic acid, inhibited degranulation induced by Ca2+ or by GTP-gamma-S but not degranulation induced by the combination of Ca2+ plus GTP-gamma-S. It is concluded that under suboptimal conditions of stimulation with Ca2+ or GTP-gamma-S, protein dephosphorylation plays an important role in the degranulation response in human neutrophils.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis
  • Antigens, Neoplasm*
  • Cell Adhesion Molecules*
  • Cell Degranulation / drug effects*
  • Ethers, Cyclic / pharmacology
  • Fluorides / pharmacology*
  • GTP-Binding Proteins / physiology
  • Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
  • Humans
  • Membrane Glycoproteins / analysis
  • Neutrophils / drug effects*
  • Neutrophils / physiology
  • Okadaic Acid
  • Permeability
  • Platelet Membrane Glycoproteins / analysis
  • Tetraspanin 30

Substances

  • Antigens, CD
  • Antigens, Neoplasm
  • CD63 protein, human
  • Cell Adhesion Molecules
  • Ethers, Cyclic
  • Membrane Glycoproteins
  • Platelet Membrane Glycoproteins
  • Tetraspanin 30
  • Okadaic Acid
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • GTP-Binding Proteins
  • Fluorides