Abstract
Four recombinant vaccinia viruses expressing different portions of the dengue type 1 virus (DEN-1) genome (C-prM-E-NS1-NS2A-NS2B; prM-E; prM-E-NS1-NS2A-NS2B; or NS1-NS2A) were constructed in order to establish the most immunogenic configuration of DEN-1 proteins. Both recombinants producing prM and E in the absence of C induced the synthesis of extracellular forms of E in vitro. Mice inoculated with these two recombinants produced DEN-1 neutralizing (NEUT) and haemagglutination inhibiting (HAI) antibodies. The other two recombinant vaccinia viruses, which did not induce the production of extracellular forms of E, did not induce E-specific immune responses. These results support our previous studies on the design of flavivirus-vaccinia vaccine candidates by showing the importance of co-expressing prM and E in order to induce the synthesis of extracellular E and to elicit NEUT and HAI antibodies.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis
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Antibodies, Viral / immunology
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Dengue / immunology
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Dengue / prevention & control
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Dengue Virus / genetics
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Dengue Virus / immunology*
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Glycoproteins / genetics
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Glycoproteins / immunology
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HeLa Cells
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Hemagglutination Inhibition Tests
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Humans
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Mice
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Neutralization Tests
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Protein Processing, Post-Translational
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Vaccinia virus / genetics*
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Vaccinia virus / immunology
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Vero Cells
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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Viral Proteins / genetics
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Viral Proteins / immunology*
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Viral Vaccines / genetics
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Viral Vaccines / immunology*
Substances
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Antibodies, Viral
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Glycoproteins
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Proteins
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Viral Vaccines
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E protein TH Sman, Dengue virus