Abstract
In the prokaryotic two-component signal transduction systems, recognition of an environmental stimulus by a sensor molecule results in the activation of its histidine kinase domain and phosphorylation of a histidine residue within that domain. This phosphate group is then transferred to an aspartate residue in the receiver domain of a cognate response regulator molecule, resulting in the activation of its output function. Although a few eukaryotic proteins were identified recently that show sequence similarity to the prokaryotic sensors or response regulators, it has not been clear whether they constituted a part of a 'two-component' system. Here we describe a two-component system in Saccharomyces cerevisiae that regulates an osmosensing MAP kinase cascade.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Histidine Kinase
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Intracellular Signaling Peptides and Proteins
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Molecular Sequence Data
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Mutation
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Phosphoprotein Phosphatases / metabolism
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Phosphorylation
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Protein Phosphatase 2
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Protein Phosphatase 2C
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Protein Tyrosine Phosphatases / genetics
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Protein Tyrosine Phosphatases / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Signal Transduction*
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Suppression, Genetic
Substances
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Fungal Proteins
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Intracellular Signaling Peptides and Proteins
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SSK1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Protein Kinases
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Histidine Kinase
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SLN1 protein, S cerevisiae
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PTC1 protein, S cerevisiae
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Phosphoprotein Phosphatases
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Protein Phosphatase 2
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Protein Phosphatase 2C
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Protein Tyrosine Phosphatases