Functional analysis of mutated cDNA clones by direct use of PCR products in in vitro transcription/translation reactions

MeSH terms

• Base Sequence
• Cloning, Molecular / methods
• DNA Primers
• DNA, Complementary / metabolism*
• DNA-Binding Proteins / biosynthesis*
• DNA-Binding Proteins / metabolism
• Escherichia coli
• Humans
• I-kappa B Proteins*
• Macromolecular Substances
• Molecular Sequence Data
• Mutagenesis, Site-Directed*
• NF-KappaB Inhibitor alpha
• NF-kappa B / biosynthesis*
• NF-kappa B / metabolism
• Oligonucleotides, Antisense
• Phosphorylation
• Point Mutation*
• Polymerase Chain Reaction / methods*
• Protein Biosynthesis
• Transcription, Genetic