Changes of NMDA receptor binding in spontaneously epileptic rat and parent strains

Neurochem Res. 1993 Nov;18(11):1169-73. doi: 10.1007/BF00978369.

Abstract

We measured the binding of [3H]3-[(+/-)2-carboxypiperazin-4-yl] propyl-1-phosphonic acid ([3H]CPP), a competitive ligand for N-methyl-D-aspartate (NMDA) receptors, in double mutant spontaneously epileptic rats (SER: zi/zi, tm/tm) and their parent strains, zitter rats and tremor rats, and WTC rats (control rats from tremor rats derived from Kyoto:Wistar rats) before and after the onset of seizures in tremor rats and SER. Significantly lower [3H]CPP binding receptor density (Bmax) was found in the cortex of SER and zitter rats at 12-15 weeks of age than in that of WTC rats and tremor rats, and at 4 weeks of age the Bmax in zitter rats was lower than that in the other strains. The reduction of Bmax in SER at 12-15 weeks of age may reflect a down regulation of NMDA receptors due to repetitive tonic seizures in SER.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding, Competitive
  • Brain / metabolism*
  • Cerebral Cortex / metabolism
  • Epilepsy / metabolism*
  • Female
  • Hippocampus / metabolism
  • Male
  • Mesencephalon / metabolism
  • Piperazines / metabolism
  • Rats
  • Rats, Mutant Strains
  • Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
  • Receptors, N-Methyl-D-Aspartate / metabolism*

Substances

  • Piperazines
  • Receptors, N-Methyl-D-Aspartate
  • 3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid