Detection of point mutations in DNA using capillary electrophoresis in a polymer network

A W Kuypers; P M Willems; M J van der Schans; P C Linssen; H M Wessels; C H de Bruijn; F M Everaerts; E J Mensink

doi:10.1016/0378-4347(93)80090-q

Detection of point mutations in DNA using capillary electrophoresis in a polymer network

J Chromatogr. 1993 Nov 24;621(2):149-56. doi: 10.1016/0378-4347(93)80090-q.

Authors

A W Kuypers¹, P M Willems, M J van der Schans, P C Linssen, H M Wessels, C H de Bruijn, F M Everaerts, E J Mensink

Affiliation

¹ Department of Internal Medicine, University Hospital Nijmegen, Netherlands.

PMID: 8294536
DOI: 10.1016/0378-4347(93)80090-q

Abstract

The use of capillary electrophoresis (CE) in a polymer network for single-strand conformation polymorphism (SSCP) is investigated. SSCP is a method to detect DNA point mutations, essential in the diagnosis of several diseases. The PCR (polymerase chain reaction) amplified p53 gene, a tumour suppressor gene known to be frequently mutated in malignant cells, was subjected to CE analysis. Two single-strand DNA fragments of 372 bp in length differing in only one nucleotide could be separated. We conclude that SSCP using CE in a polymer network is a powerful method for the detection of point mutations in DNA sequences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Capillary Action
DNA / chemistry*
DNA / isolation & purification
Electrophoresis / methods*
Genes, p53*
Humans
Molecular Sequence Data
Multiple Myeloma / genetics
Nucleic Acid Conformation
Point Mutation*
Polymerase Chain Reaction
Polymers*
Polymorphism, Genetic

Substances

Polymers
DNA