Abstract
Mycobacterium bovis BCG vaccine strains were compared with Mycobacterium tuberculosis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A 25-kDa protein observed in the BCG strains was absent in M. tuberculosis. Rabbit antibodies specific to the 25-kDa protein uniquely identified this protein in BCG strains but not in M. tuberculosis. It is suggested that the 25-kDa protein and polyclonal antibodies directed against this antigen can be exploited to distinguish BCG strains from M. tuberculosis.
MeSH terms
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Antibodies, Bacterial
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / isolation & purification
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BCG Vaccine / adverse effects
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BCG Vaccine / chemistry
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BCG Vaccine / immunology
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Bacterial Proteins / chemistry
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Bacterial Proteins / immunology
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Bacterial Proteins / isolation & purification*
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Electrophoresis, Polyacrylamide Gel
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Humans
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Immunocompromised Host
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Molecular Weight
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Mycobacterium bovis / chemistry*
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Mycobacterium bovis / immunology*
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Mycobacterium bovis / isolation & purification
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Mycobacterium tuberculosis / chemistry*
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Mycobacterium tuberculosis / immunology*
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Mycobacterium tuberculosis / isolation & purification
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Sodium Dodecyl Sulfate
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Species Specificity
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Tuberculosis / etiology
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Tuberculosis / immunology
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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BCG Vaccine
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Bacterial Proteins
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Sodium Dodecyl Sulfate