Identification of a 25-kilodalton protein of Mycobacterium bovis BCG to distinguish BCG strains from Mycobacterium tuberculosis

J Clin Microbiol. 1996 Jan;34(1):224-6. doi: 10.1128/jcm.34.1.224-226.1996.

Abstract

Mycobacterium bovis BCG vaccine strains were compared with Mycobacterium tuberculosis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A 25-kDa protein observed in the BCG strains was absent in M. tuberculosis. Rabbit antibodies specific to the 25-kDa protein uniquely identified this protein in BCG strains but not in M. tuberculosis. It is suggested that the 25-kDa protein and polyclonal antibodies directed against this antigen can be exploited to distinguish BCG strains from M. tuberculosis.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Bacterial
  • Antigens, Bacterial / chemistry
  • Antigens, Bacterial / isolation & purification
  • BCG Vaccine / adverse effects
  • BCG Vaccine / chemistry
  • BCG Vaccine / immunology
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / immunology
  • Bacterial Proteins / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immunocompromised Host
  • Molecular Weight
  • Mycobacterium bovis / chemistry*
  • Mycobacterium bovis / immunology*
  • Mycobacterium bovis / isolation & purification
  • Mycobacterium tuberculosis / chemistry*
  • Mycobacterium tuberculosis / immunology*
  • Mycobacterium tuberculosis / isolation & purification
  • Sodium Dodecyl Sulfate
  • Species Specificity
  • Tuberculosis / etiology
  • Tuberculosis / immunology

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • BCG Vaccine
  • Bacterial Proteins
  • Sodium Dodecyl Sulfate