Abstract
A novel Saccharomyces cerevisiae gene, HST1, was identified from among anonymous cDNAs and the complete corresponding genomic clone was isolated and sequenced. HST1 is very closely related to SIR2, showing 71% sequence identity over 84% of its length. Polymerase chain reaction with degenerate primers on S. cerevisiae DNA identified three additional SIR2-related genes designated HST2, HST3 and HST4. The sequences of HST2, HST3 and HST4 correspond to sequences previously released by the S. cerevisiae genome sequencing project as U33335, NCBI gi:965078; X87331, NCBI gi:829135; and Z48784, YD9346.03, respectively. Disruption of HST1 has shown no phenotype with respect to mechanisms in which SIR2 has a role, namely, regional silencing of HML alpha, or in rDNA recombination.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA Primers / genetics
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DNA, Fungal / genetics
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DNA-Binding Proteins / genetics
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Fungal Proteins / genetics*
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Gene Deletion
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Genes, Fungal*
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Histone Deacetylases*
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Humans
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Molecular Sequence Data
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Multigene Family*
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Mutation
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Phenotype
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Recombination, Genetic
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Silent Information Regulator Proteins, Saccharomyces cerevisiae*
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Sirtuin 1
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Sirtuin 2
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Sirtuins
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Trans-Activators / genetics
Substances
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DNA Primers
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Silent Information Regulator Proteins, Saccharomyces cerevisiae
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Trans-Activators
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HST1 protein, S cerevisiae
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SIR2 protein, S cerevisiae
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SIRT1 protein, human
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Sirtuin 1
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Sirtuin 2
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Sirtuins
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Histone Deacetylases
Associated data
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GENBANK/L47120
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GENBANK/M97690
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GENBANK/U33335
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GENBANK/X72225
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GENBANK/X87331
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GENBANK/Z48784