Genetic mapping and chromosome localization of the rat mitochondrial glycerol-3-phosphate dehydrogenase gene, a candidate for non-insulin-dependent diabetes mellitus

Genomics. 1996 Nov 15;38(1):96-9. doi: 10.1006/geno.1996.0599.

Abstract

The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (mtGPD) plays an important role in the regulation of insulin secretion and has been postulated as a candidate responsible for the pathogenesis of non-insulin-dependent diabetes mellitus (NIDDM) in humans as well as in rodent models of NIDDM. Recent molecular genetic studies of the Goto-Kakizaki (GK) rat model of NIDDM have identified loci linked to NIDDM. To elucidate whether rat mtGPD might play a role in the pathogenesis of NIDDM, the rat mtGPD gene (Gpd2) was cloned, and a genetic marker for Gpd2 was developed. The gene mapped to the region of rat chromosome 3 that contains a region linked to NIDDM in the GK rat. Fluorescence in situ hybridization was also carried out to verify the map position.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromosome Mapping*
  • Cloning, Molecular
  • Diabetes Mellitus, Type 2 / genetics*
  • Disease Models, Animal
  • Genetic Linkage
  • Glycerolphosphate Dehydrogenase / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Mitochondria / enzymology*
  • Molecular Sequence Data
  • Rats
  • Restriction Mapping

Substances

  • Glycerolphosphate Dehydrogenase

Associated data

  • GENBANK/U43332