Production of monoclonal antibodies against quail Pax-1

Hybridoma. 1996 Feb;15(1):83-6. doi: 10.1089/hyb.1996.15.83.

Abstract

Monoclonal antibodies (MAbs) against quail Pax-1 were generated using a fusion protein of the C-terminal part of Pax-1 and glutathione S-transferase. The MAbs generated could detect quail Pax-1 protein by Western blotting and immunoprecipitate it from whole cell extracts of quail embryos, indicating their usefulness for biochemical analyses of Pax-1 protein function in development or oncogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / chemistry
  • Blotting, Western
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / immunology*
  • DNA-Binding Proteins / isolation & purification
  • Immunoglobulin Isotypes / chemistry
  • Paired Box Transcription Factors
  • Precipitin Tests
  • Quail / genetics
  • Quail / immunology*
  • Transcription Factors / genetics
  • Transcription Factors / immunology*
  • Transcription Factors / isolation & purification

Substances

  • Antibodies, Monoclonal
  • DNA-Binding Proteins
  • Immunoglobulin Isotypes
  • Paired Box Transcription Factors
  • Transcription Factors
  • PAX1 transcription factor