There are multiple peptidoglycan hydrolases associated with Bacillus subtilis 168 and these potentially lethal enzymes have been implicated in a number of important cellular processes. Several enzymes have been studied at the molecular level and their structural genes characterized. This information has begun to identify roles for individual enzymes in motility, cell separation, differentiation, and phage lysis. It has become apparent that in many cases important autolytic functions can be performed by more than one enzyme, so the complex web of mutually compensatory components can be unraveled only by making multiple mutants. One such multiple mutant has revealed the presence of several previously unknown minor autolysins, the functions of which are currently obscure.