Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR

D A Lashkari; J H McCusker; R W Davis

doi:10.1073/pnas.94.17.8945

Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR

Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):8945-7. doi: 10.1073/pnas.94.17.8945.

Authors

D A Lashkari¹, J H McCusker, R W Davis

Affiliation

¹ Department of Genetics, Beckman Center, Stanford University, Stanford, CA 94305, USA.

Abstract

The recent ability to sequence whole genomes allows ready access to all genetic material. The approaches outlined here allow automated analysis of sequence for the synthesis of optimal primers in an automated multiplex oligonucleotide synthesizer (AMOS). The efficiency is such that all ORFs for an organism can be amplified by PCR. The resulting amplicons can be used directly in the construction of DNA arrays or can be cloned for a large variety of functional analyses. These tools allow a replacement of single-gene analysis with a highly efficient whole-genome analysis.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
DNA Primers*
Genome*
Humans
Polymerase Chain Reaction / methods*
Sequence Analysis*

Substances

DNA Primers

Abstract

Publication types

MeSH terms

Substances

Grants and funding