The white gene of the tephritid fruit fly Bactrocera tryoni is characterized by a long untranslated 5' leader and a 12kb first intron

Insect Mol Biol. 1997 Nov;6(4):343-56. doi: 10.1046/j.1365-2583.1997.00188.x.

Abstract

A 300 bp fragment from exon 6 of the white gene of Bactrocera tryoni was used to screen a B. tryoni genomic library. One positive (approximately 14 kb) insert contained exons 2-6 of white by nucleotide and amino acid sequence similarity to the white genes of D. melanogaster (O'Hare et al., 1984; Pepling & Mount, 1990). Lucilia cuprina (Garcia et al., 1996). Ceratitis capitata (Zwiebel et al., 1995) and Anopheles gambiae (Besansky et al., 1995). A white 5' cDNA fragment containing exons 1, 2 and part of exon 3 was amplified, cloned and sequenced. An inverse PCR fragment of genomic DNA was generated, containing the exon 1 coding region plus approximately 2.1 kb of upstream sequence, encompassing the putative promoter of the gene. Exon 1 was found to be 728 bp long, encoding the first twenty-five amino acids. The full length of intron 1 was shown to be 12 kb (amplified using long PCR protocols), up to 3 times the length of the longest white intron 1 isolated to date.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Diptera / genetics*
  • Drosophila Proteins*
  • Eye Color / genetics
  • Eye Proteins / genetics*
  • Genes, Insect / genetics*
  • Introns / genetics*
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics*
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription, Genetic / genetics

Substances

  • ATP-Binding Cassette Transporters
  • DNA, Complementary
  • Drosophila Proteins
  • Eye Proteins
  • w protein, Drosophila

Associated data

  • GENBANK/U97104