Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)

Genomics. 1997 Nov 1;45(3):535-40. doi: 10.1006/geno.1997.4966.

Abstract

The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase; EC 2. 4.1.119) (DDOST) catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. We isolated mouse and human DDOST cDNAs from retinoic acid-treated mouse P19 EC cells and human NT-2 cells, respectively. DDOST mRNA is expressed intensely in heart and pancreas, but at lower levels in brain. Here we show that the human DDOST 48-kDa subunit gene (HGMW-approved symbol DDOST) is organized into 11 exons expanding about 9 kb. This DDOST subunit gene is localized on chromosome 1p36.1 by fluorescence in situ hybridization analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosomes, Human, Pair 1
  • DNA, Complementary
  • Hexosyltransferases*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Membrane Proteins*
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • RNA Splicing
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transferases / genetics*

Substances

  • DNA, Complementary
  • Membrane Proteins
  • Transferases
  • Hexosyltransferases
  • dolichyl-diphosphooligosaccharide - protein glycotransferase

Associated data

  • GENBANK/D89060
  • GENBANK/D89063