Human papillomavirus detection in high-grade squamous intraepithelial lesions. Comparison of hybrid capture assay with a polymerase chain reaction system

I Nindl; D M Zahm; C J Meijer; J M Walboomers; A Schneider

doi:10.1016/0732-8893(95)00187-5

Human papillomavirus detection in high-grade squamous intraepithelial lesions. Comparison of hybrid capture assay with a polymerase chain reaction system

Diagn Microbiol Infect Dis. 1995 Dec;23(4):161-4. doi: 10.1016/0732-8893(95)00187-5.

Authors

I Nindl¹, D M Zahm, C J Meijer, J M Walboomers, A Schneider

Affiliation

¹ Department of Gynecology, Friedrich Schiller University, Jena, Federal Republic of Germany.

PMID: 9407223
DOI: 10.1016/0732-8893(95)00187-5

Abstract

The validity of human papillomavirus (HPV) detection using the hybrid capture assay (HCA) was compared with the polymerase chain reaction (PCR) in 38 patients with high-grade squamous intraepithelial lesions (HSILs). HCA and PCR showed 84% agreement for HPV detection. HCA missed a significant higher proportion of HSIL compared with PCR (21% vs. 5%; P = .04). Thus, the sensitivity of HCA should be increased before this test can be recommended for HSIL.

Publication types

Comparative Study

MeSH terms

Carcinoma, Squamous Cell / virology*
DNA, Viral / analysis
Female
Humans
Nucleic Acid Hybridization / methods*
Papillomaviridae / isolation & purification*
Papillomavirus Infections / virology*
Polymerase Chain Reaction / methods*
Reagent Kits, Diagnostic
Tumor Virus Infections / virology*
Uterine Cervical Dysplasia / virology*

Substances

DNA, Viral
Reagent Kits, Diagnostic