Abstract
Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anaphase
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Anaphase-Promoting Complex-Cyclosome
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cdc20 Proteins
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Cell Cycle Proteins*
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Chromosomes, Fungal / metabolism
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DNA Replication
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Genes, Fungal
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Kinesins / genetics
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Kinesins / metabolism
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Ligases / metabolism
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Mad2 Proteins
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Metaphase
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Mitosis*
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Mutagenesis, Site-Directed
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Mutation
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Nuclear Proteins
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Recombinant Fusion Proteins / metabolism
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Schizosaccharomyces / cytology*
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Schizosaccharomyces / genetics
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Schizosaccharomyces / metabolism*
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Schizosaccharomyces pombe Proteins*
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Spindle Apparatus / metabolism*
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Transformation, Genetic
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Ubiquitin-Protein Ligase Complexes*
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Ubiquitin-Protein Ligases
Substances
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Calcium-Binding Proteins
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Carrier Proteins
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Cdc20 Proteins
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Cell Cycle Proteins
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Cut7 protein, S pombe
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Fungal Proteins
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Mad2 Proteins
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Nuclear Proteins
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Recombinant Fusion Proteins
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Schizosaccharomyces pombe Proteins
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mad2 protein, S pombe
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slp1 protein, S pombe
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Ubiquitin-Protein Ligase Complexes
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Anaphase-Promoting Complex-Cyclosome
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Ubiquitin-Protein Ligases
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Kinesins
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Ligases