Abstract
We have examined the role of Fas and Bcl-2 in T cell survival and responses to antigen in vivo using T cells that express a transgenic antigen receptor specific for hen egg lysozyme (HEL) and that either lack functional Fas or Fas ligand (FasL) or overexpress Bcl-2 as a transgene. HEL-specific, Bcl-2-transgenic T cells showed prolonged responses to immunization with cognate peptide but were eliminated rapidly when exposed to HEL expressed systemically as a self antigen. In contrast, Fas- and FasL-defective T cells did not display exaggerated responses to immunization with HEL peptide, but did show increased expansion and survival in response to systemic self antigen and were able to activate anti-HEL (self) antibody-forming cells. Thus, Bcl-2 and Fas play different roles in the regulation of T cell responses to antigen in vivo and in self tolerance.
Publication types
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Research Support, U.S. Gov't, P.H.S.
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Retracted Publication
MeSH terms
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Adoptive Transfer
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Animals
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Apoptosis / immunology*
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Autoantibodies
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Enzyme-Linked Immunosorbent Assay
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Fas Ligand Protein
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Homeostasis
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Immune Tolerance*
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Ligands
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Lymphocyte Activation
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Membrane Glycoproteins / immunology
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Mice
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Mice, Mutant Strains
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Mice, Transgenic
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Muramidase / immunology
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Peptide Fragments / immunology
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Phenotype
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Polymerase Chain Reaction
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Proto-Oncogene Proteins c-bcl-2 / immunology*
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / immunology
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T-Lymphocytes / immunology*
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T-Lymphocytes, Helper-Inducer / immunology
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fas Receptor / immunology*
Substances
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Autoantibodies
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Fas Ligand Protein
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Fasl protein, mouse
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Ligands
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Membrane Glycoproteins
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Peptide Fragments
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Proto-Oncogene Proteins c-bcl-2
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Receptors, Antigen, T-Cell
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fas Receptor
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hen egg lysozyme peptide (46-61)
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Muramidase