Interleukin-18 activates the IRAK-TRAF6 pathway in mouse EL-4 cells

Biochem Biophys Res Commun. 1998 Mar 6;244(1):183-6. doi: 10.1006/bbrc.1998.8236.

Abstract

The pleiotropic biological activities of interleukin-18 (IL-18) are mediated by IL-18 receptor (IL-18R). When the ligand binds to the IL-18R, IL-18R initiates a signaling cascade that results in the activation of nuclear factor kappa B (NF-kappa B). When mouse EL-4 cells were exposed to IL-18, IL-1 receptor-associated kinase (IRAK) was recruited to IL-18R and was phosphorylated. In addition, tumor necrosis factor receptor-associated factor-6 (TRAF6) was associated with IRAK. Therefore, we concluded that IL-18/IL-18R-mediated signaling may share the IRAK/TRAF6 pathway through NF-kappa B activation with the IL-1/IL-1 receptor system.

MeSH terms

  • Animals
  • Carrier Proteins / metabolism*
  • Carrier Proteins / physiology
  • Cytokines / metabolism
  • Cytokines / pharmacology*
  • Enzyme Activation
  • Interleukin-1 Receptor-Associated Kinases
  • Interleukin-18
  • Interleukins / metabolism
  • Interleukins / pharmacology*
  • Mice
  • NF-kappa B / metabolism
  • Protein Kinases / metabolism*
  • Protein Kinases / physiology
  • Proteins*
  • Receptors, Interleukin-1 / metabolism*
  • Receptors, Interleukin-1 / physiology
  • Receptors, Tumor Necrosis Factor / metabolism
  • Signal Transduction / drug effects
  • T-Lymphocytes / enzymology
  • T-Lymphocytes / metabolism
  • TNF Receptor-Associated Factor 6
  • Thymoma
  • Tumor Cells, Cultured

Substances

  • Carrier Proteins
  • Cytokines
  • Interleukin-18
  • Interleukins
  • NF-kappa B
  • Proteins
  • Receptors, Interleukin-1
  • Receptors, Tumor Necrosis Factor
  • TNF Receptor-Associated Factor 6
  • Protein Kinases
  • Interleukin-1 Receptor-Associated Kinases