Previous genetic analysis showed that AICAI transformylase, IMP cyclohydrolase and GAR synthetase are encoded by purJ, purH and purD respectively, and which constitute a operon, mapped on 90 min in genetic map of Salmonella typhimurium But recent study in E. coli indicated that the genes encoding for above three enzymes only have purH and purD, without purJ gene. Report here is the DNA sequences evidence for abence of purJ gene in Salmonella typhimurium.