Abstract
The spectrum of protein tyrosine phosphatases (PTPs) expressed in bone marrow-derived murine macrophages (BMMs) was examined using reverse transcriptase-polymerase chain reaction. Ten different PTP cDNAs were isolated and in this study we focus on mDEP-1, a type III receptor PTP. Three mDEP-1 transcripts were expressed in primary macrophages and macrophage cell lines and were induced during macrophage differentiation of M1 myeloid leukemia cells. A variant mRNA was identified that encodes an alternate carboxyl-terminus and 3' UTR. The expression of mDEP-1 was down-regulated by CSF-1 (macrophage colony-stimulating factor) and up-regulated by bacterial lipopolysaccharide, an important physiological regulator of macrophage function that opposes CSF-1 action. Whole mount in situ hybridization, and immunolocalization of the protein, confirmed that mDEP-1 is expressed by a subset of embryonic macrophages in the liver and mesenchyme. mDEP-1 was also detected in the eye and peripheral nervous system of the developing embryo. Attempts to express mDEP-1 constitutively in the macrophage cell line RAW264 were unsuccessful, with results suggesting that the gene product inhibits cell proliferation.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Bone Marrow Cells / metabolism*
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Cell Differentiation
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Cell Division
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Cell Lineage
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Cloning, Molecular
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DNA, Complementary / genetics
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Eye / embryology
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Eye / metabolism
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Eye Proteins / biosynthesis
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Eye Proteins / genetics
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Fluorescent Antibody Technique, Indirect
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Gene Expression Regulation, Developmental / drug effects*
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In Situ Hybridization
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Lipopolysaccharides / pharmacology*
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Macrophage Colony-Stimulating Factor / physiology*
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Macrophages / classification
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Macrophages / metabolism*
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Male
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Nerve Tissue Proteins / biosynthesis
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Nerve Tissue Proteins / genetics
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Peripheral Nerves / embryology
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Peripheral Nerves / metabolism
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Polymerase Chain Reaction
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Protein Tyrosine Phosphatases / biosynthesis*
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Protein Tyrosine Phosphatases / genetics
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Protein Tyrosine Phosphatases / physiology
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Receptor-Like Protein Tyrosine Phosphatases, Class 3
Substances
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DNA, Complementary
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Eye Proteins
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Lipopolysaccharides
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Nerve Tissue Proteins
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RNA, Messenger
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Macrophage Colony-Stimulating Factor
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Protein Tyrosine Phosphatases
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Ptprj protein, mouse
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Receptor-Like Protein Tyrosine Phosphatases, Class 3