A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E

J Biochem. 1998 Dec 1;124(6):1072-6. doi: 10.1093/oxfordjournals.jbchem.a022223.

Abstract

The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1, 616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched beta-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex / chemistry
  • Adrenal Cortex / metabolism*
  • Amino Acid Sequence
  • Animals
  • Apolipoproteins E / chemistry
  • Apolipoproteins E / metabolism*
  • Arteriosclerosis / metabolism
  • Binding Sites
  • Blotting, Northern
  • Cholesterol / metabolism
  • Cloning, Molecular
  • Cytoplasm / metabolism
  • Humans
  • Hyperlipidemias / metabolism
  • In Situ Hybridization / methods
  • LDL-Receptor Related Proteins
  • Liver / chemistry
  • Liver / metabolism*
  • Low Density Lipoprotein Receptor-Related Protein-5
  • Male
  • Molecular Sequence Data
  • Rabbits
  • Receptors, LDL / genetics*
  • Receptors, LDL / metabolism*
  • Transcription, Genetic

Substances

  • Apolipoproteins E
  • LDL-Receptor Related Proteins
  • LRP5 protein, human
  • Low Density Lipoprotein Receptor-Related Protein-5
  • Receptors, LDL
  • Cholesterol

Associated data

  • GENBANK/AB017498
  • GENBANK/AB017499