Identification of essential residues for the catalytic function of 85-kDa cytosolic phospholipase A2. Probing the role of histidine, aspartic acid, cysteine, and arginine.
Pickard RT, Chiou XG, Strifler BA, DeFelippis MR, Hyslop PA, Tebbe AL, Yee YK, Reynolds LJ, Dennis EA, Kramer RM, Sharp JD.
Pickard RT, et al. Among authors: sharp jd.
J Biol Chem. 1996 Aug 9;271(32):19225-31. doi: 10.1074/jbc.271.32.19225.
J Biol Chem. 1996.
PMID: 8702602
Free article.
We found previously that Ser-228 is essential for enzyme activity and is likely to function as a nucleophile in the catalytic center of the enzyme (Sharp, J. D., White, D. L., Chiou, X. G., Goodson, T., Gamboa, G. C., McClure, D., Burgett, S., Hoskins, J., Sk …
We found previously that Ser-228 is essential for enzyme activity and is likely to function as a nucleophile in the catalytic center of the …