Overproduction and preliminary crystallographic study of ribonuclease H from Escherichia coli.
Kanaya S, Kohara A, Miyagawa M, Matsuzaki T, Morikawa K, Ikehara M.
Kanaya S, et al.
J Biol Chem. 1989 Jul 15;264(20):11546-9.
J Biol Chem. 1989.
PMID: 2545673
Free article.
The structural gene for the enzyme was subcloned from pSK750 (Kanaya, S., and Crouch, R. J. (1983) J. Biol. Chem. 258, 1276-1281) to make a plasmid vector pPL801, in which the gene was under the control of bacteriophage lambda PL promoter. ...
The structural gene for the enzyme was subcloned from pSK750 (Kanaya, S., and Crouch, R. J. (1983) J. Biol. Chem. 258, 1276-12 …