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Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases.
Nat Biotechnol. 2024 Jan;42(1):87-98. doi: 10.1038/s41587-023-01748-1. Epub 2023 Mar 29.
Nat Biotechnol. 2024.
PMID: 36991112
Free PMC article.
CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering.
Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg SH.
Vo PLH, et al.
Nat Biotechnol. 2021 Apr;39(4):480-489. doi: 10.1038/s41587-020-00745-y. Epub 2020 Nov 23.
Nat Biotechnol. 2021.
PMID: 33230293
Free PMC article.
Item in Clipboard
Unbiased profiling of CRISPR RNA-guided transposition products by long-read sequencing.
Vo PLH, Acree C, Smith ML, Sternberg SH.
Vo PLH, et al.
Mob DNA. 2021 Jun 8;12(1):13. doi: 10.1186/s13100-021-00242-2.
Mob DNA. 2021.
PMID: 34103093
Free PMC article.
Item in Clipboard
Selective TnsC recruitment enhances the fidelity of RNA-guided transposition.
Hoffmann FT, Kim M, Beh LY, Wang J, Vo PLH, Gelsinger DR, George JT, Acree C, Mohabir JT, Fernández IS, Sternberg SH.
Hoffmann FT, et al. Among authors: vo plh.
Nature. 2022 Sep;609(7926):384-393. doi: 10.1038/s41586-022-05059-4. Epub 2022 Aug 24.
Nature. 2022.
PMID: 36002573
Free PMC article.
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Targeted DNA integration in human cells without double-strand breaks using CRISPR RNA-guided transposases.
Lampe GD, King RT, Halpin-Healy TS, Klompe SE, Hogan MI, Vo PLH, Tang S, Chavez A, Sternberg SH.
Lampe GD, et al. Among authors: vo plh.
bioRxiv [Preprint]. 2023 Mar 18:2023.03.17.533036. doi: 10.1101/2023.03.17.533036.
bioRxiv. 2023.
PMID: 36993517
Free PMC article.
Updated.
Preprint.
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Bacterial genome engineering using CRISPR RNA-guided transposases.
Gelsinger DR, Vo PLH, Klompe SE, Ronda C, Wang H, Sternberg SH.
Gelsinger DR, et al. Among authors: vo plh.
bioRxiv [Preprint]. 2023 Mar 21:2023.03.18.533263. doi: 10.1101/2023.03.18.533263.
bioRxiv. 2023.
PMID: 36993567
Free PMC article.
Updated.
Preprint.
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Bacterial genome engineering using CRISPR-associated transposases.
Gelsinger DR, Vo PLH, Klompe SE, Ronda C, Wang HH, Sternberg SH.
Gelsinger DR, et al. Among authors: vo plh.
Nat Protoc. 2024 Mar;19(3):752-790. doi: 10.1038/s41596-023-00927-3. Epub 2024 Jan 12.
Nat Protoc. 2024.
PMID: 38216671
Review.
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Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration.
Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH.
Klompe SE, et al. Among authors: vo plh.
Nature. 2019 Jul;571(7764):219-225. doi: 10.1038/s41586-019-1323-z. Epub 2019 Jun 12.
Nature. 2019.
PMID: 31189177
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