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Protein engineering with nonstandard amino acids.
Steward LE, Chamberlin AR. Steward LE, et al. Methods Mol Biol. 1998;77:325-54. doi: 10.1385/0-89603-397-X:325. Methods Mol Biol. 1998. PMID: 9770680 Review. No abstract available.
Novel Native and Engineered Botulinum Neurotoxins.
Steward L, Brin MF, Brideau-Andersen A. Steward L, et al. Handb Exp Pharmacol. 2021;263:63-89. doi: 10.1007/164_2020_351. Handb Exp Pharmacol. 2021. PMID: 32274579 Review.
Reengineering the specificity of the highly selective Clostridium botulinum protease via directed evolution.
Dyer RP, Isoda HM, Salcedo GS, Speciale G, Fletcher MH, Le LQ, Liu Y, Brami-Cherrier K, Malik SZ, Vazquez-Cintron EJ, Chu AC, Rupp DC, Jacky BPS, Nguyen TTM, Katz BB, Steward LE, Majumdar S, Brideau-Andersen AD, Weiss GA. Dyer RP, et al. Among authors: steward le. Sci Rep. 2022 Jun 15;12(1):9956. doi: 10.1038/s41598-022-13617-z. Sci Rep. 2022. PMID: 35705606 Free PMC article.
OnabotulinumtoxinA Displays Greater Biological Activity Compared to IncobotulinumtoxinA, Demonstrating Non-Interchangeability in Both In Vitro and In Vivo Assays.
Rupp D, Nicholson G, Canty D, Wang J, Rhéaume C, Le L, Steward LE, Washburn M, Jacky BP, Broide RS, Philipp-Dormston WG, Brin MF, Brideau-Andersen A. Rupp D, et al. Among authors: steward le. Toxins (Basel). 2020 Jun 13;12(6):393. doi: 10.3390/toxins12060393. Toxins (Basel). 2020. PMID: 32545832 Free PMC article.
Plasma membrane localization signals in the light chain of botulinum neurotoxin.
Fernández-Salas E, Steward LE, Ho H, Garay PE, Sun SW, Gilmore MA, Ordas JV, Wang J, Francis J, Aoki KR. Fernández-Salas E, et al. Among authors: steward le. Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3208-13. doi: 10.1073/pnas.0400229101. Epub 2004 Feb 24. Proc Natl Acad Sci U S A. 2004. PMID: 14982988 Free PMC article.
Novel chimeras of botulinum neurotoxins A and E unveil contributions from the binding, translocation, and protease domains to their functional characteristics.
Wang J, Meng J, Lawrence GW, Zurawski TH, Sasse A, Bodeker MO, Gilmore MA, Fernández-Salas E, Francis J, Steward LE, Aoki KR, Dolly JO. Wang J, et al. Among authors: steward le. J Biol Chem. 2008 Jun 20;283(25):16993-7002. doi: 10.1074/jbc.M710442200. Epub 2008 Apr 8. J Biol Chem. 2008. PMID: 18400760 Free article.
24 results