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Application of ultrasensitive digital ELISA for p24 enables improved evaluation of HIV-1 reservoir diversity and growth kinetics in viral outgrowth assays.
Kuzmichev YV, Lackman-Smith C, Bakkour S, Wiegand A, Bale MJ, Musick A, Bernstein W, Aronson N, Ake J, Tovanabutra S, Stone M, Ptak RG, Kearney MF, Busch MP, Wonderlich ER, Kulpa DA. Kuzmichev YV, et al. Sci Rep. 2023 Jul 6;13(1):10958. doi: 10.1038/s41598-023-37223-9. Sci Rep. 2023. PMID: 37414788 Free PMC article.
A cohort-based study of host gene expression: tumor suppressor and innate immune/inflammatory pathways associated with the HIV reservoir size.
Dwivedi AK, Gornalusse GG, Siegel DA, Barbehenn A, Thanh C, Hoh R, Hobbs KS, Pan T, Gibson EA, Martin J, Hecht F, Pilcher C, Milush J, Busch MP, Stone M, Huang ML, Reppetti J, Vo PM, Levy CN, Roychoudhury P, Jerome KR, Hladik F, Henrich TJ, Deeks SG, Lee SA. Dwivedi AK, et al. PLoS Pathog. 2023 Nov 29;19(11):e1011114. doi: 10.1371/journal.ppat.1011114. eCollection 2023 Nov. PLoS Pathog. 2023. PMID: 38019897 Free PMC article.
A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir.
Levy CN, Hughes SM, Roychoudhury P, Reeves DB, Amstuz C, Zhu H, Huang ML, Wei Y, Bull ME, Cassidy NAJ, McClure J, Frenkel LM, Stone M, Bakkour S, Wonderlich ER, Busch MP, Deeks SG, Schiffer JT, Coombs RW, Lehman DA, Jerome KR, Hladik F. Levy CN, et al. Cell Rep Med. 2021 Apr 12;2(4):100243. doi: 10.1016/j.xcrm.2021.100243. eCollection 2021 Apr 20. Cell Rep Med. 2021. PMID: 33948574 Free PMC article.