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Modification of the catalytic subunit of bovine heart cAMP-dependent protein kinase with affinity labels related to peptide substrates.
Bramson HN, Thomas N, Matsueda R, Nelson NC, Taylor SS, Kaiser ET. Bramson HN, et al. J Biol Chem. 1982 Sep 25;257(18):10575-81. J Biol Chem. 1982. PMID: 6286662 Free article.
The regeneration of catalytic activity on treatment of the modified enzymes with dithiothreitol and the observation that prior reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) blocks the modification of the kinase by these reagents are consistent with the proposal …
The regeneration of catalytic activity on treatment of the modified enzymes with dithiothreitol and the observation that prior reaction with …