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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1946 1
1952 2
1953 1
1954 2
1955 4
1956 5
1957 4
1958 7
1959 8
1960 18
1961 13
1962 45
1963 141
1964 207
1965 221
1966 266
1967 330
1968 438
1969 485
1970 496
1971 479
1972 602
1973 572
1974 588
1975 621
1976 590
1977 551
1978 510
1979 563
1980 529
1981 557
1982 518
1983 527
1984 506
1985 569
1986 493
1987 507
1988 554
1989 552
1990 612
1991 702
1992 669
1993 813
1994 896
1995 1036
1996 1126
1997 1311
1998 1470
1999 1619
2000 1824
2001 2173
2002 2328
2003 2534
2004 2954
2005 3309
2006 3748
2007 3923
2008 4115
2009 4451
2010 4716
2011 5164
2012 5540
2013 5582
2014 5737
2015 5713
2016 5702
2017 5498
2018 5256
2019 5497
2020 5907
2021 5723
2022 5470
2023 5123
2024 2366
2025 1

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121,872 results

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Page 1
Showing results for ligase a
Search for Lygas A instead (2 results)
Structural Diversity of Ubiquitin E3 Ligase.
Toma-Fukai S, Shimizu T. Toma-Fukai S, et al. Molecules. 2021 Nov 4;26(21):6682. doi: 10.3390/molecules26216682. Molecules. 2021. PMID: 34771091 Free PMC article. Review.
Three enzymes are required for achieving the ubiquitination reaction: ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3). E3s play a pivotal role in selecting substrates. Many structural studies have been conducted to reveal the …
Three enzymes are required for achieving the ubiquitination reaction: ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), a …
E3 ligase ligand chemistries: from building blocks to protein degraders.
Sosič I, Bricelj A, Steinebach C. Sosič I, et al. Chem Soc Rev. 2022 May 10;51(9):3487-3534. doi: 10.1039/d2cs00148a. Chem Soc Rev. 2022. PMID: 35393989 Review.
These heterobifunctional compounds are comprised of a protein-targeting ligand, an appropriate linker, and a ligand binding to the E3 ligase of choice. A successful PROTAC induces the formation of a ternary complex, leading to the E3 ligase-mediated ubiquitination o …
These heterobifunctional compounds are comprised of a protein-targeting ligand, an appropriate linker, and a ligand binding to the E3 lig …
RNA ligase ribozymes with a small catalytic core.
Nomura Y, Yokobayashi Y. Nomura Y, et al. Sci Rep. 2023 May 26;13(1):8584. doi: 10.1038/s41598-023-35584-9. Sci Rep. 2023. PMID: 37237056 Free PMC article.
Here, we explored simple and small ribozyme motifs capable of ligating two RNA fragments in a template-directed fashion (ligase ribozymes). One-round selection of small ligase ribozymes followed by deep sequencing revealed a ligase ribozyme motif comprising a …
Here, we explored simple and small ribozyme motifs capable of ligating two RNA fragments in a template-directed fashion (ligase riboz …
Assembling Multiple Fragments: The Gibson Assembly.
Avilan L. Avilan L. Methods Mol Biol. 2023;2633:45-53. doi: 10.1007/978-1-0716-3004-4_4. Methods Mol Biol. 2023. PMID: 36853455
This method is based on the assembly of overlapping fragments, generally produced by PCR, and then combining them using three enzymes: a 5' exonuclease, a DNA polymerase, and a DNA ligase, in an isothermal reaction. Here, we describe this method, including the design of pr …
This method is based on the assembly of overlapping fragments, generally produced by PCR, and then combining them using three enzymes: a 5' …
Horizontal acquisition of a DNA ligase improves DNA damage tolerance in eukaryotes.
Nicolas E, Simion P, Guérineau M, Terwagne M, Colinet M, Virgo J, Lingurski M, Boutsen A, Dieu M, Hallet B, Van Doninck K. Nicolas E, et al. Nat Commun. 2023 Nov 22;14(1):7638. doi: 10.1038/s41467-023-43075-8. Nat Commun. 2023. PMID: 37993452 Free PMC article.
A phylogenetic analysis reveals its orthology to prokaryotic DNA ligase E, and its horizontal acquisition by bdelloid rotifers and plausibly other eukaryotes. The fungus Mortierella verticillata, having a single copy of this DNA Ligase E homolog, also exhibits an in …
A phylogenetic analysis reveals its orthology to prokaryotic DNA ligase E, and its horizontal acquisition by bdelloid rotifers and pl …
Systemwide disassembly and assembly of SCF ubiquitin ligase complexes.
Baek K, Scott DC, Henneberg LT, King MT, Mann M, Schulman BA. Baek K, et al. Cell. 2023 Apr 27;186(9):1895-1911.e21. doi: 10.1016/j.cell.2023.02.035. Epub 2023 Apr 6. Cell. 2023. PMID: 37028429 Free PMC article.
Cellular repertoires of SCF (SKP1-CUL1-F box protein) ubiquitin ligase complexes, which mediate much protein degradation, require CAND1 to distribute the limiting CUL1 subunit across the family of 70 different F box proteins. ...The CAND1-SCF conformational ensemble recycl …
Cellular repertoires of SCF (SKP1-CUL1-F box protein) ubiquitin ligase complexes, which mediate much protein degradation, require CAN …
DNA ligase III: a spotty presence in eukaryotes, but an essential function where tested.
Simsek D, Jasin M. Simsek D, et al. Cell Cycle. 2011 Nov 1;10(21):3636-44. doi: 10.4161/cc.10.21.18094. Epub 2011 Nov 1. Cell Cycle. 2011. PMID: 22041657 Free PMC article. Review.
DNA ligases are crucial for most DNA transactions, including DNA replication, repair, and recombination. Recently, DNA ligase III (Lig3) has been demonstrated to be crucial for cell survival due to its catalytic function in mitochondria. ...
DNA ligases are crucial for most DNA transactions, including DNA replication, repair, and recombination. Recently, DNA ligase …
Vypal2: A Versatile Peptide Ligase for Precision Tailoring of Proteins.
Zhang D, Wang Z, Hu S, Lescar J, Tam JP, Liu CF. Zhang D, et al. Int J Mol Sci. 2021 Dec 31;23(1):458. doi: 10.3390/ijms23010458. Int J Mol Sci. 2021. PMID: 35008882 Free PMC article.
Owing to their mild aqueous reaction conditions, enzymatic methods based on the use of peptide ligases are particularly desirable. In this regard, the recently discovered peptidyl Asx-specific ligases (PALs) have emerged as powerful biotechnological tools in recent …
Owing to their mild aqueous reaction conditions, enzymatic methods based on the use of peptide ligases are particularly desirable. In …
In vitro protein ubiquitination assay.
Zhao Q, Liu L, Xie Q. Zhao Q, et al. Methods Mol Biol. 2012;876:163-72. doi: 10.1007/978-1-61779-809-2_13. Methods Mol Biol. 2012. PMID: 22576094
Ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3) are the three key enzymes in this process. To detect the specificity between E2 and E3 or enzyme-substrate relationship between E3 and a substrate protein, ubiquitination activit …
Ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3) are the three key enzymes in this proc …
121,872 results
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