Abstract
An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic analysis of an isolate from one member of the family. This analysis involved the use of rapid, bench-top DNA sequencing technology, open-source data release, and prompt crowd-sourced analyses. In less than a week, these studies revealed that the outbreak strain belonged to an enteroaggregative E. coli lineage that had acquired genes for Shiga toxin 2 and for antibiotic resistance.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Bacterial Typing Techniques
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Child
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Diarrhea / epidemiology
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Diarrhea / microbiology
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Escherichia coli Infections / microbiology*
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Feces / microbiology
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Female
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Genome, Bacterial*
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Germany
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Hemolytic-Uremic Syndrome / epidemiology
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Hemolytic-Uremic Syndrome / microbiology*
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Humans
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Male
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Molecular Sequence Data
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Phylogeny
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Plasmids / genetics
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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Shiga-Toxigenic Escherichia coli / classification
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Shiga-Toxigenic Escherichia coli / genetics*
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Shiga-Toxigenic Escherichia coli / isolation & purification
Associated data
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GENBANK/AFOG01000000
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GENBANK/AFVR00000000
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GENBANK/AFVS00000000